Blue (1) Features. Checkpoint Friendly (1) FastTrack Eligible (2) Decoration Method. Print (2) Life In Motion. Sort By. of 2. View. 40; 80; Life in Motion® Compact Cargo Box. Item #: X; X; As Low As $ | Add to Compare; Add to Flyer; Check Inventory; Clearance. Life in Motion® 3 Piece Travel Case Set. Life in Motion 5 Roots for Culture 6 Higher Ground (Lenzman Remix) 7 Invisible Sounds 8 Blue 9 Roarganic Symphony 10 Bad Bonus 11 Welcome to the Stateside 12 The Avenue 13 Late Night Connection 14 Take the Reigns 15 Pursuit Life in Motion. Laptop Messenger Bag w/ " Padded Sleeve - Life in Motion. $ $ Add to Cart. Laptop Backpack w/ Zip Pockets - Life in Motion Primary. $ $ Add to Cart. Large Trunk Organizer w/ Dividers - Collapsible - Life in Motion. $ $
Home Purified Mouse Anti-Calnexin. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Analyte Specific Reagent. Analytical and performance characteristics are not established. Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Product Details Recommended Assay References. Description Calnexin is a 90 kDa integral membrane protein located primarily in the ER.
Format Format Purified. Bioimaging 1. Tubulin is labeled with Tubulin Tracker Green dyeplasma membrane is labeled with CellMask Orange dyeand nucleus is stained blue with Hoechst reagent. Keeping cells alive and healthy during various experimental manipulations, detection, and imaging is no small task. The choice of medium is particularly important for time-lapse imaging and experiments where cells are exposed to ambient conditions for longer periods.
For reliable results with live cells, it is essential that the cells be healthy and kept in an environment as close as possible to physiological temperature, pH, oxygen level, and other conditions.
Learn more about cell culture. These media and wash buffers are created specifically for live-cell imaging and detection. Employing them in your experiments can help you improve image clarity, reduce background fluorescence, and optimize cell viability. You can improve image clarity, reduce background fluorescence, and optimize cell viability by using media and wash buffers created specifically for live-cell imaging and detection.
See product selection guide. The appropriate fluorophore targeted fluorescent protein or small membrane-permeant reagent should be used to monitor your target cellular structure or process.
Additional fluorophores can be used to monitor multiple cellular structures and processes, but the excitation and emission spectra should be checked using the Fluorescence SpectraViewer to ensure minimal spectra overlap. It is critical to avoid using too much fluorescent label because excessive fluorescent labeling can result in:.
Signal-to-background ratio can be optimized by using reagents that reduce extracellular fluorescence and increase fluorophore photostability.
It is important to image in media that have been specifically designed for maintaining cell health while reducing or eliminating background fluorescence in live-cell imaging experiments see Table 1. The addition of a background suppressor compatible with live cells can also help reduce extracellular background fluorescence and eliminate the need for a wash step.
Antifade mounting media for live cells can be applied to samples to reduce photobleaching of fluorophores, preventing signal loss with multiple or long exposures. Both labels show high off-cell background when the probe is left in the staining solution left. Addition of BackDrop Background Suppressor greatly reduces extracellular background while leaving intracellular labeling unaffected rightthus enabling a no-wash protocol for high-contrast imaging of tubulin in live cells.
The overall signal protection offered by ProLong Live reagent compared to untreated samples is calculated based on the scan number where treated and untreated samples reach the EC50 value. To minimize phototoxicity, choose imaging systems that give you the greatest control of light sources.
Try to minimize light intensity, exposure time, wavelength range, and amount of excitation energy for illuminating your cells while still generating a good signal with low background. Use the illumination that gives you the highest signal with the lowest level of fluorophore excitation. In some cases particularly when you wish to image over a long period of timeit is advisable to sacrifice resolution by using shorter exposure times or lower magnification in exchange for healthier cells.
Live-cell imaging over longer periods of time can be challenging because the target may move out of focus during the course of the experiment. Many microscopes have autofocusing features that can help keep your target in focus longer and reduce focal drift. Additionally, maintaining cells at a constant temperature and keeping the volume of solution in the vessel constant will help with focal drift.
Many cells cannot tolerate deviations from their optimal temperature, osmolarity, pH, and humidity. Requirements vary depending on what experimental question you are asking. For example, experiments investigating cell growth and division may have a different set of requirements than experiments involving receptor activation and calcium accumulation. Some robust immortalized cell lines will tolerate being imaged or monitored for short periods of time without any environmental control.
Conversely, for long-term imaging and detection studies, good results with both immortalized cells and primary cells typically require tightly controlled environmental parameters. A The illuminated area was subjected to repeated illumination for 10 hours. Cells in this area show signs of phototoxicity a loss of viability as cells were not able to grow into the wound. B Cells in the non-illuminated area show viable cell growth into the wound.
The top cell shows catastrophic blebbing of the cell membrane caused by excessive light exposure. Blebbing is a term used to describe membrane perturbation caused by toxicity. By contrast, the bottom cell remains relatively healthy and is not displaying aberrant morphology. To avoid the pitfall of proceeding to the next step in your experiment with unhealthy cells, a quick check for cell health can be done on the Countess II FL Automated Cell Counter when used in conjunction with a variety of fluorescent reagents to detect cell viability, apoptosis, cytotoxicity, and transfection efficiency.
The reusable slide option reduces consumption cost. Designed specifically for Invitrogen EVOS imaging systemsthe Invitrogen EVOS Onstage Incubator is an environmental chamber that enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and nonphysiological conditions. Data gathered from longer-term imaging studies are the basis of quantitative analysis studies, especially when combined with Thermo Scientific HCS Studio Software for increased statistical power.
Invitrogen cell health assays have shown excellent results on the Thermo Scientific Varioskan LUX Multimode Microplate Reader equipped with gas module, which can read a well plate in as little as six seconds.
Color your way through 30 fantastic illustrations inspired by actual cell images submitted by researchers around the world. Download the free coloring book that helps inspire your creative scientific expression. Request now. Search Thermo Fisher Scientific. Search All. See Navigation. Featured webinar.
Best practices: 5 steps to live-cell imaging. Image gallery for live-cell imaging. It was based on the invention of the magnetic tape by Fritz Pfleumerwhich uses similar technology but with open reels for which the tape was manufactured by BASF. These instruments were very expensive and relatively difficult to use and were therefore used mostly by professionals in radio stations and recording studios.
By1 million U. Despite the multiple versions, it failed. Later the similar Elcaset also failed in the market. Consumer use of magnetic tape machines took off in the early s, after playback machines reached a comfortable, user-friendly design. This was aided by the introduction of transistors which replaced the bulky, fragile, and costly vacuum tubes of earlier designs.
Reel-to-reel tape then became more suitable for household use, but still remained an esoteric product. Grundig came up with the DC-International derived from blue prints of the Compact Cassette inbut failed on the demand of distributing companies. InPhilips invented the Compact Cassette medium for audio storageintroducing it in Europe on 30 August at the Berlin Radio Show       and in the United States under the Norelco brand in Novemberwith the trademark name Compact Cassette.
By overrecorders had been sold in the US alone and Japan soon became the major source of recorders. By85 manufacturers had sold over 2. In the early years sound quality was mediocre, but it improved dramatically by the early s when it caught up with the quality of 8-track tape and kept improving. The mass production of "blank" not yet recorded Compact Cassettes began in in HanoverGermany.
The initial offering consisted of 49 titles. However, the system had been designed initially for dictation and portable use, with the audio quality of early players not Life In Motion - КГБ (Cheghebe) - Blue (Cassette) suited for music. Some early models also had an unreliable mechanical design. Inthe Advent Corporation introduced their Model tape deck that combined Dolby type B noise reduction and chromium IV oxide CrO 2 tape, with a commercial-grade tape transport mechanism supplied by the Wollensak camera division of 3M Corporation.
This resulted in the format being taken more Life In Motion - КГБ (Cheghebe) - Blue (Cassette) for musical use, and started the era of high fidelity cassettes and players. Although the birth and growth of the cassette began in the s, its cultural moment took place during the s and s. Like the transistor radio in the s and s, the portable CD player in the s, and the MP3 player in the s, the Walkman defined the portable music market for the decade of the '80s, with cassette sales overtaking those of LPs.
To prevent this, retailers would place cassettes inside oversized "spaghetti box" containers or locked display caseseither of which would significantly inhibit browsing, thus reducing cassette sales. Apart from the purely technological advances cassettes brought, they also served as catalysts for social change. Their durability and ease of copying helped bring underground rock and punk music behind the Iron Curtaincreating a foothold for Western culture among the younger generations.
One of the most famous political uses of cassette tapes was the dissemination of sermons by the Ayatollah Khomeini throughout Iran before the Iranian Revolutionin which Khomeini urged the overthrow of the regime of the ShahMohammad Reza Pahlavi.
In s India, cassettes were blamed for bringing unwanted Christian and Islamic influences into traditionally Sikh and Hindu areas. Cassette technology was a booming market for pop music in Indiadrawing criticism from conservatives while at the same time creating a huge market for legitimate recording companies, as well as pirated tapes.
While offering also mainstream music, the "gas station cassettes" became associated with genres like Gipsy rhumbalight music and joke tapes that were very popular in the 's and 's. Between andthe cassette tape was the most popular format in the UK and record labels experimented with innovative packaging designs.
A designer during the era explained: "There was so much money in the industry at the time, we could try anything with design. Untilcassettes remained the dominant medium for purchasing and listening to music in some developing countriesbut compact disc CD technology had superseded the Compact Cassette in the vast majority of music markets throughout the world by this time.
In Western Europe and North America, the market for cassettes declined sharply after its peak in the late s. This was particularly noticeable with pre-recorded cassettes, the sales of which were overtaken by those of CDs during the early s. Since then, further decline occurred, with very few retailers stocking them because they are no longer issued by the major music labels.
Sales of pre-recorded music cassettes in the U. Cassettes remained popular for specific applications, such as car audio and telephone answering machineswell into the s.
Cassettes players were typically more resistant to shocks than CD players, and their lower fidelity was not considered a serious drawback. However, the advent of "shock proof" buffering technology in CD players, the general heightening of consumer expectations, and the introduction of CD auto-changers meant that, by the early s, the CD player rapidly replaced the cassette player as the default audio component in the majority of new vehicles in Europe and America.
As of [update]blank cassettes were still being produced and sold at some retail stores, often identified as audio cassettes, with the original Compact Cassette logo all but vanished from the packaging.
Cassette recorders and players were gradually becoming more scarce, and fewer facilities for cassette duplication remained available. While digital voice recorders are now common, microcassette recorders are still used for note taking in business and educational settings. Audiobookschurch services, and other spoken-word material are sometimes sold on cassette. Although portable digital recorders are most common today, analog tape remains a desirable option for certain artists and consumers.
Even among major label stars, the form has at least one devotee: Thurston Moore claimed in"I only listen to cassettes. InBotswana-based Diamond Studios announced plans  for establishing a plant to mass-produce cassettes in a bid to combat piracy.
It opened in In South Korea, the early English education boom for toddlers encourages a continuous demand for English language cassettes, as of [update]due to the affordable cost. Inthe Oxford English Dictionary removed the word "cassette player" from its 12th edition Concise version. In India, film and devotional music continued to be released in the cassette format due to its low cost until National Audio Company in Missouri, the largest of the few remaining manufacturers of audiocassettes in the U.
Inretail chain Urban Outfitterswhich had long carried Vinyl LPsstarted carrying a line of new pre-recorded cassette tapes along with blank cassettes and players  featuring both new and vintage albums. A number of synthwave artists such as The MidnightMichael Oakley and Anders Enger Jensen have released their albums on cassette in addition to the usual digital download format.
Some tracks in this genre notably The Midnight's Endless Summer and MemoriesLeBrock's Please Don't Cry and Michael Oakley's Rabbit in the Headlights include sound effects at the beginning or end of the track to help give an ambience of listening on cassette even when the music itself is being played digitally.
Sincecassette tape sales have seen a modest resurgence, withand all showing increased sales. The cassette was a great step forward in convenience from reel-to-reel audio tape recordingalthough, because of the limitations of the cassette's size and speed, it initially compared poorly in quality. Unlike the 4-track stereo open-reel format, the two stereo tracks of each side lie adjacent to each other, rather than being interleaved with the tracks of the other side.
This permitted monaural cassette players to play stereo recordings "summed" as mono tracks and permitted stereo players to play mono recordings through both speakers. The tape is 0. In stereo, each track is further divided into a left and a right channel of 0. Cassette tapes are made of a polyester-type plastic film with a magnetic coating.
The original magnetic material was based on gamma ferric oxide Fe 2 O 3. Circa3M Company developed a cobalt volume-doping process combined with a double-coating technique to enhance overall tape output levels. This product was marketed as "High Energy" under its Scotch brand of recording tapes. For this reason, some low-grade IEC Type I tapes have been marketed specifically as better suited for data storage than for sound recording.
In  DuPontthe inventor of chromium dioxide CrO 2 manufacturing process, began commercialization of CrO 2 media. The first CrO 2 cassette was introduced in by Advent and later strongly backed by BASFthe inventor and longtime manufacturer of magnetic recording tape. Cobalt - adsorbed iron oxide Avilyn was introduced by TDK in and proved very successful.
The tape coating on most cassettes sold today as either "normal" or "chrome" consists of ferric oxide and cobalt mixed in varying ratios and using various processes ; there are very few cassettes on the market that use a pure CrO 2 coating. Simple voice recorders and earlier cassette decks are designed to work with standard ferric formulations.
Newer tape decks usually are built with switches and later detectors for the different bias and equalization requirements for higher grade tapes, Life In Motion - КГБ (Cheghebe) - Blue (Cassette). The recording bias levels also were different. The quality normally is reflected in the price; Type I cassettes generally are the cheapest, and Type IV are usually the most expensive. BASF chrome tape used in commercially pre-recorded cassettes used type I equalization to allow greater high-frequency dynamic range for better sound quality, but the greater selling point for the music labels was that the Type I cassette shell could be used for both ferric and for chrome music cassettes.
Notches on top of the cassette shell indicate the type of tape. Type I cassettes have only write-protect notches, Type II have an additional pair next to the write protection ones, and Type IV metal have a third set near the middle of the top of the cassette shell. These allow later cassette decks to detect the tape type automatically and select the proper bias and equalization.
An exception to this standard were mechanical storytelling dolls from the s e. These toys used the Type IV notches to detect that a specially coded tape had been inserted, where the audio of the story is stored on the left channel and various cue tones to tell the doll's servos how and when to move along with the story on the right channel. Tape length usually is measured in minutes of total playing time.
The most popular varieties always marketed with a capital letter 'C' prefix are C46 23 minutes per sideC60 30 minutes per sideC90, and C The C46 and C60 lengths typically are 15 to 16 micrometers 0. Some vendors are more generous than others, providing or meters or feet rather than meters feet of tape for a C90 cassette.
Even C tapes were available at one time,  but these were extremely thin and fragile and suffered from such effects as print-throughwhich made them unsuitable for general use. All of these were discontinued - Maxell simplified its cassette offer to 10, 20, 60 and minute lengths, Sony exited the audio cassette market globally, and Imationlicensee of the TDK trademark, exited the consumer products market. Some companies included a complimentary blank cassette with their portable cassette recorders in the early s.
Panasonic 's was a C14 and came with a song recorded on side one, and a blank side two. Except for C74 and C, such non-standard lengths always have been hard to find, and tend to be more expensive than the more popular lengths. Home taping enthusiasts may have found certain lengths useful for fitting an album neatly on one or both sides of a tape.
For instance, the initial maximum playback time of Compact Discs was 74 minutes, explaining the relative popularity of C74 cassettes. The full tape width is 3. For mono recording the track width is 1. In stereo mode each channel has width of 0. The "head gap" of a tape recorder refers to the space, in the direction of tape movement, between the ends of the pole pieces of the head.
Without a gap the head would produce a "closed" magnetic field and so would not interact sufficiently with the magnetic domains on the tape. A narrower gap would give a higher frequency limit but also weaker magnetization.
Separate record and playback heads were already a standard feature of more expensive reel-to-reel tape machines when cassettes were introduced, but their application to cassette recorders had to wait until demand developed for higher quality reproduction, and for sufficiently small heads to be produced.
All cassettes include a write protection mechanism to prevent re-recording and accidental erasure of important material. Each side of the cassette has a plastic tab on the top that may be broken off, leaving a small indentation in the shell.
This indentation allows the entry of a sensing lever that prevents the operation of the recording function when the cassette is inserted into a cassette deck. If the cassette is held with one of the labels facing the user and the tape opening at the bottom, the write-protect tab for the corresponding side is at the top-left. Occasionally and usually on higher-priced cassettes, manufacturers provided a movable panel that could be used to enable or disable write-protect on tapes.
If later required, a piece of adhesive tape can be placed over the indentation to bypass the protection, or on some decksthe lever can be manually depressed to record on a protected tape. Extra care is required to avoid covering the additional indents on high bias or metal bias tape cassettes adjacent to the write-protect tabs.
In most cassettes, the magnetic tape is attached to each spool with a leader, usually made of strong plastic. This leader protects the weaker magnetic tape from the shock occurring when the tape reaches the end. Leaders can be complex: a plastic slide-in wedge anchors a short fully opaque plastic tape to the take-up hub; one or more tinted semi-opaque plastic Life In Motion - КГБ (Cheghebe) - Blue (Cassette) follow; the clear leader a tintless semi-opaque plastic segment follows, which wraps almost all the way around the supply reel, before splicing to the magnetic tape itself.
The clear leader spreads the shock load to a long stretch of tape instead of to the microscopic splice. Various patents have been issued detailing leader construction and associated tape player mechanisms to detect leaders.
The disadvantage with tape leaders is that the sound recording or playback does not start at the beginning of the tape, forcing the user to cue forward to the start of the magnetic section. For certain applications, such as dictation, special cassettes containing leaderless tapes are made, typically with stronger material and for use in machines that had more sophisticated end-of-tape prediction.
Home computers that made use of cassettes as a more affordable alternative to floppy discs e. Some cassettes were made to play a continuous loop of tape without stopping.
Lengths available are from around 30 seconds to a standard full length. They are used in situations where a short message or musical jingle is to be played, either continuously or whenever a device is triggered, or whenever continuous recording or playing is needed. Some include a sensing foil on the tape to allow tape players to re-cue. From as early as various patents have been issued, covering such uses as uni-directional, bi-directional, and compatibility with auto-shut-off and anti-tape-eating mechanisms.
One variant has a half-width loop of tape for an answering machine outgoing message, and another half-width tape on spools to record incoming messages. Cassette tape adapters allow external audio sources to be played back from any tape player, but were typically used for car audio systems.
An attached audio Life In Motion - КГБ (Cheghebe) - Blue (Cassette) with a phone connector converts the electrical signals to be read by the tape head, while mechanical gears simulate reel to reel movement without actual tapes when driven by the player mechanism. This feature each includes a rail to guide the tape to the spool and prevent an unclean roll from forming. The competition responded by inserting additional deflector pins closer to the coils in the lower plastic case half.
Some low-priced and pre-recorded compact cassettes were made without pulleys; the tape is pulled directly over the capstan drive. Cassette playback suffered from some flaws frustrating to both professionals and home recording enthusiasts. Tape speed could vary between devices, resulting in pitch that was too low or too high. Speed often was calibrated at the factory, and could not be changed by users. The slow tape speed increased tape hiss and noise, and in practice delivered higher values of wow and flutter.
Different tape formulation and noise reduction schemes artificially boosted or cut high frequencies and inadvertently elevated noise levels. Noise reduction also adds some artifacts to the sound, which a trained ear can hear sometimes quite easily.
Wow and Flutter, however, can sometimes be added intentionally to recordings for aesthetic reasons. See Lo-fi music. A common mechanical problem occurred when a worn-out or dirty player rotated the supply spool faster than the take-up spool or failed to release the heads from the tape upon ejection.
This would cause the magnetic tape to be fed out through the bottom of the cassette and become tangled in the mechanism of the player. In these cases the player was said to have "eaten" or "chewed" the tape, often destroying the playability of the cassette. The first cassette machines e. Early machines required attaching an external dynamic microphone. Most units from the s onwards also incorporated built-in condenser microphones, which have extended high-frequency response, but may also pick up noises from the recorder's motor.
A portable recorder format still common today is a long box, the width of a cassette, with a speaker at the top, a cassette bay in the middle, and "piano key" controls at the bottom edge. Another format is only slightly larger than the cassette, known popularly as the "Walkman" a Sony trademark.
View credits, reviews, tracks and shop for the Cassette release of Cell Therapy on Discogs. Label: LaFace Records - • Format: Cassette Single • Country: US • Genre: Hip Hop •/5(5). Due to the special care and handling required to fulfill your Rush Service order, the following conditions must be met. Complete order must be placed online with payment, or received no later than PM EST email to [email protected]; Complete and accurate electronic or camera-ready artwork must be received no later than PM EST to the Rush Service email address: rushorders. Updated: 16/11/ Related article: Speeds – rear sprockets. This post will explain bicycle cassette compatibility i.e. what cassettes can be mixed with which rear shifters/faharderimarneusobisecocontge.co compatibility with various chains, see: Chain compatibility post. Overview of compatibility and possible combinations will be given according to the number of sprockets.
flywheel is in motion. • Do not turn the crank arms by hand. • Never face backward while using a LifeCycle GX exercise bike. • This unit is NOT equipped with a freewheel system. If the flywheel is in motion, the pedals will be in motion. Do not attempt to stop the unit by applying reverse pressure to the pedals as knee injury may occur.
Shimano XTR CS-M Speed Cassette $ - $ This button adds this product to a list of products that can be used to make comparisons between all the products in the list. About Life Fitness For more than 45 years, Life Fitness has been dedicated to creating fitness solutions that benefit both facilities and exercisers. Our mission to keep people active started with the Lifecycle exercise bike and continues with the widest range of cardio, strength and Reviews:
Description. Calnexin is a 90 kDa integral membrane protein located primarily in the ER. It contains a long N-terminal calcium-binding domain that extends into the lumen of the ER and a .
flywheel is in motion. • Do not turn the crank arms by hand. • Never face backward while using a LifeCycle GX exercise bike. • This unit is NOT equipped with a freewheel system. If the flywheel is in motion, the pedals will be in motion. Do not attempt to stop the unit by applying reverse pressure to the pedals as knee injury may occur. Description. Calnexin is a 90 kDa integral membrane protein located primarily in the ER. It contains a long N-terminal calcium-binding domain that extends into the lumen of the ER and a .
Protocol for Life Balance is a professional brand. Their products are formulated to be administered by recommendation and under the supervision of a healthcare professional. Doctor Trusted Brand ; average based on product reviews. Filter Results. Filter by Name. Category. Aches and Pains (20).
Jibba Jabba - The Basstards (2) - EP (Vinyl), Come Into My World - Kylie Minogue - This Is Me... (CD), The Spoon - To Separate The Flesh From The Bones - Utopia Sadistica (CD, Album), Barynya - Various - Диско-Клуб 13(Б) (Vinyl, LP), Nocturian Victory (Instrumental), Orpheus In The Underworld - Various - Concert Favorites (Best Known Light Classics) (Vinyl, LP), Vul Bujhe Chole Jao - Rinku - Anurager Manush (CD, Album), Mother - Bigz - A New Big-Inning (CD), Debu Liar - Zainal Abidin - Zainal Abidin (CD, Album), Orange Trumpet - Various - Add To Friends (CD), For So Long (Original Mix) - Dave Horne - Artist Focus 22 (File, MP3), San Antonio Rose - The Kai Warner Singers And Orchestra* - Dance Party (Vinyl, LP), Sweat (Extended Mix) - The Cut (3) & Mind Electric - Sweat (Vinyl), Do Not Utter A Word - Lou Caimano, Girl, I Want You - Various - Gravel Volume 5 (CD)